Expressing:Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer.
Article Snippet: All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.. Cell lines and culture conditions The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Article Snippet: The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Immunofluorescence:Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer.
Article Snippet: All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.. Cell lines and culture conditions The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Article Snippet: The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Staining:Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer.
Article Snippet: All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.. Cell lines and culture conditions The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Article Snippet: The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Fluorescence:Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer.
Article Snippet: All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.. Cell lines and culture conditions The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Article Snippet: The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Quantitative RT-PCR:Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer.
Article Snippet: All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.. Cell lines and culture conditions The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Article Snippet: The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Migration:Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer.
Article Snippet: All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.. Cell lines and culture conditions The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Article Snippet: The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
In Vitro:Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer.
Article Snippet: All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.. Cell lines and culture conditions The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Article Snippet: The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Western Blot:Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer.
Article Snippet: All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.. Cell lines and culture conditions The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Article Snippet: The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Invasion Assay:Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer.
Article Snippet: All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.. Cell lines and culture conditions The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Article Snippet: The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Clone Assay:Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Anti-colorectal cancer effects of IRX4 and sensitivity studies to oxaliplatin
Article Snippet: The normal human intestinal epithelial cell line FHC and CRC cell lines HCT116, SW480, HCT8, and Caco2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).. The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA) (Cat No. 10370021).The cell culture conditions were as follows: FHC and SW480 cell lines were cultured in GibcTM Dulbecco’s Modified Eagle Medium (DMEM) high-glucose medium (Cat No. 11965092), HCT116 and HCT-8 cell lines in GibcoTM RPMI 1640 medium (Cat No. 11875093), and Caco2 cells in GibcoTM Minimum Essential Medium (MEM) (with NEAA)..
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer.
Article Snippet: All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.All patients provided written informed consent before surgery, and our study were approved by the Ethics Committee of the First Affiliated Hospital of Zunyi Medical College according to the 1975 Declaration of Helsinki.. Cell lines and culture conditions The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
Article Title: Gab2 facilitates epithelial-to-mesenchymal transition via the MEK/ERK/MMP signaling in colorectal cancer
Article Snippet: The human CRC cell lines HT29, SW480, SW620 and LOVO, and a normal human intestinal epithelial cell line FHC were obtained from the American Type Culture Collection (ATCC, Manassas, USA), and authenticated according to the ATCC recommendations.. SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.SW480 and SW620 cells were cultured in Leibovitz’s L-15 medium (GIBCO Laboratories, Grand Island, NY, USA) supplemented with 10 % fetal bovine serum (FBS) (HyClone, Logan, UT, USA), 100 U/ml penicillin and 100 μg/ml streptomycin.
|
ATCC is a verified supplier 
ATCC manufactures this product 
